Oil Red in Propylene Glycol Method

EMS26504

Components:

Oil Red O Solution
Mayer's Haematoxylin
Harris' Haematoxylin
Propylene Glycol 85% Aqueous Solution
Hydrochloric Acid 5%
Ammonia Water 0.3%

Fixation:    

10% Buffered Neutral Formalin

Section:      

Frozen

Staining Procedure:

1.  Cut frozen section and collect in distilled water.
2.  Propylene Glycol, Absolute, for 2 minutes
3.  Oil Red O Solution, for one hour. If sections are mounted on glass slides before staining, allow staining process to continue overnight.
4. Differentiate in Propylene Glycol, 85%, for one minute. Rinse in two changes of distilled water.
5. Stain for a few seconds in Mayer’s Haematoxylin, or Harris’s Haematoxylin. Rinse in two changes of distilled water.
6. If over-stained differentiate in Hydrochloric Acid, 5%, wash in water; neutralise in Ammonia Water, 0.3%.
7. Wash in two changes of distilled water. Mount with Glycerin Jelly.

Results:

              Fat = Red

              Nuclei = Blue

References:

 AFIP Manual of Histologic Staining Methods, Ed. L. Luna, 3rd ed.,

 New York, McGraw-hill Publications, c. 1968, p. 140.