EMS26173
Fixation
Formalin-Acetone Fixative
Procedure
- Fix thin blood films in Formalin Acetone Fixative for 15 seconds.
- Rinse gently in distilled water.
- Stain in 3-Amino-9-Ethylcarbazole staining solution for 2.5 minutes.
- Wash in running tap water.
- Stain in Mayer's Haematoxylin for 8 minutes.
- Wash in tap water, air dry, and mount in Glycerine Jelly.
Stain Results
Sites of peroxidase activity | Red-Brown granules |
Nuclei | Blue |
References
Willams, W. J., Beutler, E., Erslev, A.J. and Rundles, R.W. Hematology , 2 nd ed.McGraw-Hill , NY , c 1977, pp1627-1628.
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