EMS6090-05, 26090-06; 26090-20; 26090-25
Staining Protocol
- Deparaffinise and hydrate to distilled water.
- Stain in filtered Congo Red, 1% Aqueous for 1 hour.
Note: If using a microwave, stain in Congo Red, 1% Aqueous for 25 seconds. The hot stain jar is removed from the oven and should sit at room temperature for 1 minute.
- Rinse in distilled water.
- Quickly differentiate in Alkaline Alcohol.
- Wash in running tap water for approximately 5 minutes.
- Counterstain in Mayer's Acid Hemalum for approximately 5 minutes.
Note: If using a microwave, oven-stain for 30 seconds.
- Wash in running tap water for 15 minutes.
- Dehydrate and clear through 95% ethyl (absolute alcohol changes each), 2 minutes each.
- Mount using resinous medium.
Stain Results
Amyloids | Pink Red |
Nuclei | Blue |
Elastic Tissue | Light Red |
Was this article helpful?
That’s Great!
Thank you for your feedback
Sorry! We couldn't be helpful
Thank you for your feedback
Feedback sent
We appreciate your effort and will try to fix the article