Alcoholic Congo Red Solution

Created by Kathleen Patrick, Modified on Fri, 1 Nov at 9:10 AM by Nicole Smith

EMS6090-05, 26090-06; 26090-20; 26090-25

 

Staining Protocol

  1. Deparaffinise and hydrate to distilled water.
  2. Stain in filtered Congo Red, 1% Aqueous for 1 hour.

Note: If using a microwave, stain in Congo Red, 1% Aqueous for 25 seconds. The hot stain jar is removed from the oven and should sit at room temperature for 1 minute.

  1. Rinse in distilled water.
  2. Quickly differentiate in Alkaline Alcohol.
  3. Wash in running tap water for approximately 5 minutes.
  4. Counterstain in Mayer's Acid Hemalum for approximately 5 minutes.

Note: If using a microwave, oven-stain for 30 seconds.

  1. Wash in running tap water for 15 minutes.
  2. Dehydrate and clear through 95% ethyl (absolute alcohol changes each), 2 minutes each.
  3. Mount using resinous medium.

 

Stain Results

Amyloids

Pink Red

Nuclei

Blue

Elastic Tissue

Light Red

 

 

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