Lacquers's Method for Alcoholic Hyalin

Created by Kathleen Patrick, Modified on Thu, 7 Nov at 2:59 PM by Kathleen Patrick

EMS26306

Components

Mayer's Haematoxylin

Acid-Fuchsin Aniline Solution

Light Green 1% Aqueous

Phosphomolybdic Acid 1%

Alcoholic Picric Acid

Fixation:

10% Buffered Neutral Formalin or Zenker’s

Sections:

Cut Paraffin, 6µm

Staining Procedures:

Deparaffinize and hydrate to distilled water.
Stain in Mayer Haematoxylin for 15 minutes. Wash in running water for 15 minutes.
Flood slides with Acid Fuchsin-Aniline Solution. Shake bottle well before using, solution will separate upon standing, and heat gently until aniline fumes escapes, view against a dark background. Let stand for 15 minutes. Wash in water, 3 dips.
Differentiate in Alcoholic Picric Acid until only hyaline and red cells remain red, and collagen is faint gray or unstained. Wash in running water for 3 minutes.
Mordant in 1% Phosphomolybdic Acid for 4 to 18 hours.
Counterstain in Light Green Solution for 1 hour. Wash in running water for 1 minute.
Fibers are seen as distinct fibrils.
Dehydrate in 95% alcohol, absolute alcohol, and clear in xylene, two changes each.
Mount with Permount.

Stain Results:

Mallory Bodies	Bright Red
Erythrocytes	Red
Cystoplasm	Pale Brown
Bile Pigment	Green
Protinaceous material seen occasionally in some liver cells	Red
Hemosiderin and lipofichsin	Unstained

References:

Lacquer, G.I., Amer. J. Clin. Path., 20:689-690, 1950.

Luna, L.G. (ed), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw-Hill, NY, c 1968

Lillies, R.D. Fullmer, H.M., Histopathologic Technic and Pratical Histochemistry 4th ed., McGraw-Hill, N.Y. 1976, pp 670-671.

Clark, G., (ed). Staining Procedures, 3rd edition, Williams & Wilkins, Baltimore, p. 52, c.1973.