Modified Verhoeff Elastic-Van Gieson Stain

Created by Kathleen Patrick, Modified on Thu, 7 Nov at 4:30 PM by Kathleen Patrick

EMS26369


Components

Alcoholic Haematoxylin

Ferric Chloride 2% aqueous

Lugol’s Iodine

Ferric Chloride 0.4% aqueous

Van Geisons Solution

Consistent and shorter staining time!


Fixation

10% Buffered Neutral Formalin


Sections

4-5µm


Staining

  1. Deparaffinise and hydrate sections.
  2. Stain in modified Verhoeff at room temperature for 7 minutes or in a 60°C oven for 3.5 minutes.
    To prepare modified Verhoeff Working Elastic solution, mix just before use:
    • 3 parts Alcoholic Haematoxylin, 3%
    • 2 parts Ferric Chloride 2%, aqueous
    •  1 part Lugol's Iodine
  3. Wash in running warm tap water for 1 minute.
  4. Differentiate in Ferric Chloride, 0.4% aq. for 75 seconds.
  5. Wash in running warm tap water for 5 minutes.
  6. Counterstain in Van Geisons solution for 60 seconds.
  7. Dehydrate, clear and mount with Permount.


Results

Nuclei

Greyish-black

Elastic Fibres

Bluish-black

Collagen fibres

Red

Muscle

Greenish yellow


References

Garvey, Winsome et al "A modified Verhoff Elastic Van Geison Stain" The Journal of Histotechnology, Vol. 14, No. 2 pp113-114 June 1991.

 

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