EMS26321
Fixation:
Absolute Alcohol 4 – 8 hours.
Sections:
Paraffin or Celloidin
Staining Procedures:
- Deparaffinise and hydrate to distilled water.
- Place on staining rack and rinse several times with distilled water.
- Place 2 ml of Mayer Mucihematein, Modified on each slide. Allow to remain for five minutes. Drain off stain and wash well with distilled water three times for 5 minutes each.
- Dehydrate in two changes of 95% alcohol, two changes of absolute alcohol, Absolute Alcohol-Xylene (50:50), and two changes of Xylene.
- Mount with Permount or DPX.
Stain Results:
Mucin | Deep Violet |
Cell Nuclei | Pale Grey-Blue |
Connective Tissue | Pale Grey to Colourless |
References:
Laskey, Alice: Stain Techn., 25:33 (1950).
Clark G.: Staining Procedures, William & Wilkins Company, Baltimore, 3rd Ed., c. 1973, p. 47
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