EMS26356
Components:
Resorcin-Fuchsin Solution
Nuclear Fast Red Solution
Periodic Acid 0.5% Aqueous
Sodium Bisulphite Solution
Fixation:
Carnoy’s Solution
Sections:
Paraffin at 6µm
Staining Procedures:
- Deparaffinise and hydrate to distilled water.
- Periodic Acid, 0.5% for 5 minutes. Rinse in two changes of distilled water.
- Sodium Bisulphite Solution for 15 hours. Rinse in five or six changes of distilled water.
- Resorcin-Fuchsin Solution for 4 hours> Rinse in three changes of distilled water.
- Counterstain in Nuclear Fast Red for 4 hours. Rinse in 3 changes of distilled water.
- Dehydrate in 95% Alcohol, Absolute Alcohol, and clear in Xylene two changes each.
- Mount with Permount or DPX.
Stain Results:
Basement Membranes | Black in cross section/Grey in tangential sections |
Nuclei | Pink to Red |
References:
Puchtler, H. and Sweat, F.: Stain Techn., 39:163, 1964.
AFIP Manual of Histological Staining Methods, 3rd ed., ED. L. Luna: New York: McGraw Hill Publications, c. 1968, p. 73
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