USER NOTES
Differential Quik III Stain Kit (Modified Giemsa) -
User Notes (DG)
Introduction
The Differential Quik III Stain Kit is a high quality, rapid turnaround time staining kit that serves many purposes in the cost-conscious laboratory environment. The Differential Quik III Stain Kit may be used for fine needle aspirations, cytology, blood smears, bone marrow biopsies as well as bone marrow smears, touch preps, various microorganisms, as well as detecting the H. Pylori Microorganism. The kit contains a fixative for air-dried cell suspensions and/or touch preparation slides.
Applications
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Kit Contents
Fixative – teal
Solution I – red
Solution II – blue
Procedure
Fixative: Frozen sections, touch preps, bone marrow biopsies and blood smears for 10 seconds in Fixative
Dip 5 times in Solution I
Dip 5 times in Solution II
Wash in Distilled water and air dry
Quickly dehydrate in 100% EtOH, then xylene and mount in a synthetic resin
Results
Structure
| Colour
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Erthrocytes | Pink/yellowish red |
Platelets | Dark blue/violet/purple granules |
Neutrophils | Blue nucleus, pink cytoplasm, purple granules |
Eosinophils | Blue nucleus, blue cytoplasm, red granules |
Basophils | Dark blue/purple nucleus, purple granules |
Monocytes | Purple nucleus, light blue cytoplasm |
Bacterial/Microorganisms | Deep blue, vary in shape |
Collagen, cytoplasm & muscle | Various shades of pink, blue, orange and yellow |
Storage and Handling
Storage: Store refrigerated 0-5°C - Transport at room temperature
Safety: Refer to SDS for details
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