EMS26096-25 - Differential Quik III Stain Kit (Modified Giemsa)

Created by Nicole Smith, Modified on Wed, 07 Dec 2022 at 09:47 AM by Nicole Smith

EMS Catalog # 26096-25, 26096-50 & 26096-75

The Differential Quik III Stain Kit is a high quality, rapid turnaround time staining kit that serves many purposes in the cost-conscious laboratory environment. The Differential Quik III Stain Kit may be used for fine needle aspirations, cytology, blood smears, bone marrow biopsies as well as bone marrow smears, touch preps, various microorgan-isms, as well as detecting the H.Pylori Microorganism. The kit contains a fixative for air-dried cell suspensions and/or touch preparation slides.

Applications:

Fine needle aspirations
Frozen Sections
Blood Smears
Cytological specimens
Cytopreps
Bone marrow biopsy
Touch preps
Microorganisms

Kit Contents:

Fixative -Teal
Solution I- Red/Orange
Solution II - Blue

Storage and handling:

Storage: Room Temperature
Safety: Refer to the MSDS for details.

Procedure:

Fixative: Fix frozen sections, touch preps, bone marrow biopsies and blood smears for 10 seconds inFixative.
Solution I-Red: Dip 5 times in Solution I
Solution II-Blue: Dip 5 times in Solution II
Wash in Distilled water and air dry.
Quickly dehydrate in 100% EtOH,then xylene and mount in a synthetic resin.

Results:

Structure	Color
Erthrocytes	Pink/yellowish red
Platelets	Dark blue/violet/purple granules
Neutrophils	Blue nucleus, pink cytoplasm, purple granules
Eosinophils	Blue nucleus, blue cytoplasm, red granules
Basophils	Dark blue purple nucleus, purple granules
Monocytes	Purple nucleus, light blue cytoplasm
Bacteria/microorganisms	Deep blue, vary in shape
Collagen, cytoplasm & muscle	Various shades of pink, blue, orange & yellow