May-Grunwald Giemsa For Helicobactor Pylori

EMS26114

Components

Jenner’s Stain Stock Solution

Giemsa Stock Solution

Acetic Acid 0.1% Solution

Fixation

10% Buffered Neutral Formalin

Sections

3µm

Procedure

1. Deparaffinise and hydrate to distilled water.
2. Place promptly in methanol (methyl alcohol), two changes, three times each.
3. Stain in Jenner's Working solution for 6 minutes.
   To prepare working solution mix:
   • 1 part Jenner's Stain
   • 1 part Distilled water
    Prepare fresh!
4. Stain in Working Giemsa solution for 10 minutes.
   To prepare working solution mix:
   • 2.4ml Giemsa Stock
   • 50ml Distilled water
    Prepare fresh-do not reuse!
5. Differentiate in Acetic Acid, 0.1% (A-114-3) one slide at a time, gently agitate, checking often under microscope until defined.
    DO NOT OVER DIFFERENTIATE!
6. Dehydrate quickly in tert-Butyl Alcohol to Xylene
7. Mount.

Stain Results

References

Carson, F. Histotechnology: A Self-Instructional Text . 1980-109.