Giemsa for Helicobactor Pylori and Mast Cells

EMS26110

Components

Methanol

Giemsa Stock Solution

Acetic Acid 1%

Fixation

10% Buffered Neutral Formalin

Sections

3µm

Procedure

1. Deparaffinise and hydrate to distilled water.
2. Place promptly in Methanol for 2 minutes.
3. Preheat Working Geimsa solution in microwave on high for 45 seconds. Filter the hot solution into a coplin jar containing the slides. Stain for 2 minutes.
   To prepare working solution mix:
   • 7.0ml Geimsa Stock
   • 38ml Distilled water
   • 5ml Methanol
    Prepare fresh- do not reuse!!
4. Rinse quickly in distilled water
5. Dip one slide at a time in Acetic Acid 1% .
6. Rinse quickly in distilled water.
7. Rinse in 3 changes of Methanol.
8. Clear in Xylene and mount.

Stain Results

NOTE: The use of methanol to dehydrate stained sections prior to mounting seems to provide lighter staining of surrounding tissue, offering better contrast with stained organisms.

References

Helicobacter pylori in peptic ulcer disease, NIH Consensus Statement Online, January 1994, 12 (1): 1:23 . Luna l AFIP 3 rd ed. McGraw Hill, 236.

Fail, Rena , AS , HT (ASCP) Medical University of South Carolina , Histologic . Vol. XXXIV, No. 2, Nov. 2001. P 32.