Ehrlich’s Haematoxylin

EMS26753

Components:

Ehrlich's Haematoxylin Solution

Acid Alcohol 1%

Sodium Acetate 1% Aqueous

Ammonium Water 0.3%

Lithium Carbonate, Saturated

Fixation:

Formalin 10% Neutral Buffered or fixative of choice

Sections:

Paraffin @ 5-6µm

Staining Procedures:

1. Deparaffinise and hydrate to distilled water.
2. Stain for 2 – 5 minutes in Ehrlich’s Haematoxylin. Wash in distilled water.
3.  Differentiate in Acid Alcohol until the sections turn red.
4. Blue section in Sodium Acetate 1% or Ammonium Water or Lithium Carbonate Solution.
5. Wash thoroughly and counterstain.

References:

P. Ehrlich "Fragekasten" ["Questionbox"] Zeitschr. für Wiss. Mikr. Techn. III,I:150, 1886. Article in German.

Mallory, F.B., Pathological Technique. W.B. Saunders Co, Philadelphia, p. 73, 1938.

Clark, G., (ed). Staining Procedures, 3rd edition, Williams & Wilkins, Baltimore, pp 33-34, 1973.

Luna, L.G. (Ed), Manual of Histologic Staining Methods of the AFIP, 3rd Edition, McGraw-Hill, NY, pp 35 and 38, c. 1968.