Oil Red O Method For Lipofuchsin

EMS26609

Components

Oil Red O Solution 0.5%

Propylene Glycol 85%  Aqueous

Mayer’s Haematoxylin

Fixation

10% Buffered Neutral Formalin

Section

Paraffin at 6µm

Staining Procedure

1. Deparaffinise and hydrate to distilled water.
2. Stain in Oil Red O Solution for 72 hours.
3. Agitate the slide in Propylene Glycol, 85% for 1 minute.
4. Two changes of distilled water, 2 minutes each
5. Stain in Mayer's Haematoxylin for 5 minutes. Wash in running water for 10 minutes.
6. Mount with Glycerin Jelly

Staining Results

References

Luna, L.G. (ed.), Manual of Histologic Staining Methods of the AFIP , 3 rd edition, The AFIP, 3 rd edition, McGraw-Hill, NY, P. 186, c 1968.