Herxheimer Technique for Fat

EMS26500

Components:

Sudan IV Staining Solution

Harris' Haematoxylin

Acetic Acid 1% Aqueous

Fixation:

Formalin

Sections:

Frozen

Staining Procedures:

1. Immerse in 70% Alcohol for an instant.
2. Stain for 2 – 5 minutes in Sudan IV Staining Solution.
3. Wash quickly in 70% Alcohol. Rinse in distilled water.
4. Counterstain in Harris Haematoxylin. Follow with 2 – 5 minutes in Acetic Acid, 1% for clearer blue staining of nuclei.
5. Wash thoroughly in tap water.
6. Mount (Glycerin or Glycerin Jelly.

Stain Results:  

References:

Clark, G, ed.: Staining Procedures, 3rd ed.: Baltimore: Williams & Wilkins Co., 1973, p. 143

Conn’s, H.J.: Biological Stains; 8th Ed., Baltimore: Williams and Wilks Co., c. 1969, p. 100