Relative Acidophilia Stain

EMS26901

Components

Phloxine B Staining Solution

Tartrazine Saturated Solution In Cellosolve

N-Butyl Alcohol

Fixation

Formalin Solutions

Sections

Paraffin

Staining (Use 5 Slides)

1. Deparaffinise and hydrate to distilled water.
2. Stain for all 5 slides in Phioxine B, 0.5% Solution for 30 minutes.
3. Remove one slide, rinse in water and 95% alcohol and hold in n-Butyl Alcohol. Rinse the remaining 4 slides quickly in water.
4. Stain the remaining 4 slides in Tartrazine Solution and remove at 2,5, 10 and 20 minutes. Each time, rinse in 95% alcohol and hold in n-Butyl Alcohol following removal.
5. When all 5 slides are in n-Butyl Alcohol, change to fresh n-Butyl Alcohol for 4 minutes, then clear and mount.

Staining Results

The least acidophilic elements stained in the phloxine control slide lose the red colour after 2 minutes in the tartrazine solution. The most acidophilic retain the phloxine after 20 minutes in the tartrazine.

Reference

Lendrum, A.C., J. Path. Bact ., 59:399-404, 1947.

Clark ,G., (ed.), Staining Procedures , 3 rd edition, Williams & Wilkins, Baltimore , p. 163, c 1973.