Harris' Haematoxylin (1900)

EMS26754

Haematoxylin with or without mercury

Components:

Harris' Haematoxylin Solution

Harris' Haematoxylin Without Mercury

Acid Alcohol 1%

Sodium Acetate 1% Aqueous

Ammonia Water 0.3%

Lithium Carbonate Saturated Solution

Fixation:

Formalin 10% Neutral Buffered or fixative of choice

Sections:

Paraffin @ 5-6µm

Staining Procedures:

1. Deparaffinise and hydrate to distilled water
2.  
   1. Stain in Harris' Haematoxylin containing 2-4ml Glacial Acetic Acid per 100ml for 5 – 15 minutes. Wash in water.
   2. If using Harris; Haematoxylin without Mercury containing 2-4ml Glacial Acetic Acid per 100ml for 5 – 15 minutes. Wash in water.
3. Differentiate in Acid Alcohol until the sections turn red.
4. Blue section in Sodium Acetate, 1% or Ammonium Water or Lithium Carbonate Solution.
5. Wash thoroughly and counterstain.

References:

Harris, H.F., J. Appl. Microscopy, III: 777-781, 1900.

Mallory, F.B., Pathological Technique. W.B. Saunders Co, Philadelphia, 1938.

Clark, G., (ed). Staining Procedures, 3rd edition, Williams & Wilkins, Baltimore, pp 33-35, 1973.

Luna, L.G. (Ed), Manual of Histologic Staining Methods of the AFIP, 3rd Edition, McGraw-Hill, NY, pp 34 and 38, c. 1968.