Toren's Method For Mast Cells

EMS26472

Components

Giemsa Stain Solution

Acetic Acid, O.5%, Aqueous

Pollacks’s Trichrome Solution

Fixation

10% Buffered Neutral Formalin

Section

Paraffin, @ 6µm

Staining Procedure

1. Deparaffinise and hydrate to distilled water.
2. Stain in *Working Giemsa Solution for 75 minutes. Rinse in distilled water.
   ***Prepare Working Giemsa Solution by adding 40 drops of
   Giemsa Stock Solution to 40 ml of distilled water and mixing thoroughly.
    Prepare fresh, immediately before use.
3. Place in Acetic Acid 0.5% for 5 to 10 seconds. Rinse in distilled water.
4. Stain in Pollack's Trichrome Solution for 10 to 15 seconds.
5. Rinse in Acetic Acid 0.5% until no more stain washes off, 3 to 5 seconds.
6. Dehydrate in 95% Alcohol, Absolute Alcohol and clear in Xylene.
7. Mount with Permount.

Results

References

Toren, D.A.: Stain Techn ., 38:249-250, 1963.

Luna, L., (ed.), Manual of Histologic Staining Methods of the AFIP , 3 rd . ed.,
 McGraw-Hill , New York , c. 1968, pp. 116-117.