Truant's Fluorescent Method For Acid Fast Organisms

EMS26102

Components

Weigert’S Iron Haematoxylin Solution A

Weigert’S Iron Haematoxylin Solution B

Auramine-Rhodamine Staining Solution

Acid Alcohol 1%

Fixation

10% Buffered Neutral Formalin

Section

Paraffin, @ 6µm

Staining Procedure

Use control slide

1. Deparaffinise and hydrate to distilled water.
2. Weigert's Iron Hematoxylin solution for 10 minutes. Prepare working Weigert's Haematoxylin by mixing 1 vol. of Weigert's Haematoxylin A, with 1 vol. of Weigert's Haematoxylin B. Wash in running water for 10 minutes.
3. Auramine-Rhodamine Solution, @ 60 °C for 10 minutes, or 37 °C for 15 minutes, or 25 °C for 20 minutes. Wash in tap water.
4. Remove excess stain with Acid Alcohol, 1%, for 2-3 minutes. Wash in running water. Rinse in distilled water.
5. Dehydrate in 95% alcohol, absolute alcohol, and clear in Xylene, two changes each.
6. Mount with Permount.

Results

References

Traunt, J.P. Henry Ford Hospital Med. Bull., 10:287, 1962.

AFIP Manual of Histological Staining Methods, 3 rd ed. , Ed. L. Luna: New York : McGraw Hill
 Publications, c. 1968, p. 219.

Clark, G.: Staining Procedures, Williams and Wilkins Co., Baltimore , 3 rd Ed., c. 1973, p. 225.