Harris' Haematoxylin

EMS26041

Fixation

Follow most fixatives.

Sections

Paraffin

Staining

1. Deparaffinise and hydrate to distilled water..
2. Stain in Harris Haematoxylin containing 2 – 4ml of Glacial Acetic Acid per 100ml for 5-15 minutes. Wash in water.
3. Differentiate in Acid Alcohol until the sections turn red.
4. Blue section in Sodium Acetate, 1% or Ammonia Water, 0.3%, or Lithium Carbonate Solution
5. Wash thoroughly and counterstain.

References

Harris, H.F., J.  Appl. Microscopy, III: 777-781, 1900

Mallory, F. B., Pathological Technique, W. B. Saunders Co., Philadelphia, 1938.

Clark, G., (ed.), Staining Procedures, 3rd Edition, William & Wilkins, Baltimore, pp33 & 35, c 1973.

Luma, L.G., (ed.) Manual of Histologic Staining Method of the AFIP, 3rd edition, McGraw-Hill, NY, pp 34 & 38, c1968.