Technovit 9100 MMA Embedding Kit

Components

• Technovit 9100 Basic Solution - Component 1 SDS
  The Technovit 9100 basic solution is comprised of stabilised methyl methacrylate. The hydrophily is improved through the addition of a suitable hydrolysing agent. Technovit 9100 basic solution can be used when stabilised and unstabilised.
• Technovit 9100 PMMA Powder - Component 2  SDS
  The PMMA powder is used to guarantee a clear decrease in polymerisation shrinkage, a reduction in the polymerisation heat released and a better polymerisation process.
• Technovit 9100 Hardener 1 - Component 3 SDS
  Hardening powder 1 is a peroxide compound that starts polymerisation with hardener 2.
• Technovit 9100 Hardener 2 - Component 4 SDS
  Hardening liquid 2 acts as a catalyst for hardener 1 to facilitate targeted polymerisation even at very low temperatures [< 0°C].
• Technovit 9100 Regulator - Component 5  SDS
  This is comprised of a reactive organic compound that facilitates a regulated polymerisation with controlled low temperature spikes even for large quantities of polymerisation.
• PMMA-Granulate, EXART  SDS
  This granulate acts as an additional internal filler when larger amounts (500-1000 ml) of polymer are to be used, for example, in the case of femur shaft with noncemented endoprosthses. The amount of monomer (basic solution) is thereby reduced, at the same time making the polymerization easier to control.

Application:

Fixation - tissue pre-treatment

Fixation is done for 12 to 24 hours in various fixation solutions depending on the size of the tissue and the antigen/enzyme to be detected. Over fixation must always be avoided.

The following fixation methods are possible for detecting antigens/enzymes:

1. 4% neutral buffered formalin solution (0.1M phosphate or 0.02M phosphate buffer for iliac crest biopsies)
2. 10% buffered formalin solution (0.1 M phosphate buffer)
3. Fixation solution in accordance with Schaffer(formol/alcohol)
4. 1.4% paraformaldehyde solution, cold (+4 to +8°C) for 24 - 28 hours (sensitive enzyme detection such as alkaline phosphatase, fixation-sensitive antigens)

Dehydration, intermedium and immersion (pre-infiltration 1-3, infiltration)