EMS26614
Components:
Silver Nitrate 5%
Methenamine 30%
Sodium Borate 5%
Gold Chloride 0.1%
Sodium Thiosulfate 3%
Light Green Working Solution
Fixation:
Absolute Alcohol at 4°C for overnight.
Sections:
Paraffin @ 4-5µm
Staining Procedures:
- Deparaffinise to Xylene and rinse with 3 changes of absolute alcohol.
- Place slides in preheated working* Methenamine Silver. Incubate for 30 minutes @60°C.
To prepare working solution:
Methenamine-Silver Nitrate Stock Solution:
Silver Nitrate, 5% - 5ml
Methenamine, 3% - 100ml
Methenamine-Silver Nitrate Working Solution
Methenamine Silver-Nitrate Stock Solution - 25ml
Distilled water - 25ml
Sodium Borate, 5% - 2ml
Prepare fresh prior to use. DO NOT USE IF CLOUDY. Urate Crystals should be black
- Rinse sections in distilled water.
- Tone with Gold Chloride, 0.1% for 5 minutes.
- Give sections 4 or 5 rinses of distilled water.
- Sodium Thiosulfate, 3% for 5 minutes.
- Wash in tap water for 5 minutes.
- Rinse in distilled water.
- Counterstain in Light Green Working Solution for 2 minutes.
- Dehydrate, clear and mount with Permount or any synthetic resin
Stain Results:
Urate Crystals | Black |
Background | Green |
References:
Sheehan, Denza C. and Hrapchak, Barbara B. Theory and Practice of Histotechnology, 2nd ed. The C.V. Mosby Company, St. Louis, p.225 – 226, c. 1980.
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