Pearse's Method for Phospholipids

Created by Kathleen Patrick, Modified on Fri, 8 Nov at 8:19 AM by Kathleen Patrick

EMS26516


Components:

Luxol Fast Blue MBS Solution 0.1%

Lithium Carbonate 0.05% Aqueous

Neutral Red Solution 1% Aqueous


Fixation:

Bakers Calcium Cadmium Formal.


Sections:

Paraffin @ 6µm


Staining Procedures:

  1. Deparaffinise and place in absolute alcohol.
  2. Stain in Luxol Fast Blue Solution overnight at 58°C. Rinse in 70% Alcohol and then rinse in distilled water.
  3. Differentiate for 30 minutes to 2 hours in Lithium Carbonate 0.05%. Rinse in distilled water.
  4. Counterstain for 30 minutes in Neutral Red, 1%. Rinse in distilled water and blot dry.
  5. Dehydrate in 95% alcohol, absolute alcohol and clear in xylene, two changes, two minutes each change.
  6. Mount with Permount


Stain Results:  

Phospholipids

Blue

Nuclei and Nucleoli

Red

Early Lipofuchin, Eosinophil, Granules, Keratin, Keratohyalin, Human Elastic Tissues

Blur to Purple


References:

Pearse, A.G.E., J. Path. Bact., 70:554-557, 1995.

Luna, L.G. (ed). Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 3rd ed., McGraw-Hill, N.Y., c. 1968, p.148

 

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