The terms ocular and eyepiece mean the same thing.
The terms reticle, reticule and graticule are used interchangeably by various organisation and countries, they all are referring to the same thing.
It is not normal to have a calibration certificate for an eyepiece reticle. The reason for this is that the reticle is installed in the eyepiece and all of the optics on the microscope can effect the size measured by the reticle. It is normal practice to use a stage micrometer to calibrate the complete microscope.
Size conversions and equivalents.
1mm = 1000µm | 1inch = 1000thou | 1mm = 39.4thou |
0.1mm = 100µm | 0.1inch = 100thou | 1thou = 0.0254mm (25.4µm) |
0.01mm = 10µm | 0.01inch = 10thou | |
0.001mm = 1µm |
It is far better to increase the objective magnification than use a reticle with finer details. A recent example had a customer that wanted to have a reticle with a horizontal scale to measure spores of 2‐5µm in diameter. They said they normally used a 10x objective. We answered as follows;
'In this case, our NE1, which has a 10mm scale subdivided into 0.1mm divisions will measure 0‐1mm when used with a 10x objective lens. Each division will represent 0.01mm (10um). This will not be good enough to measure spores in the 2um ‐ 5um region. If you use a reticle with a smaller scale in the eyepiece it is very difficult to read so the answer is for the customer to use the 40x objective. Now the 10mm scale will measure 0‐0.25mm and each division will represent 2.5um. If the customer is able to use the 100x objective then the measurements can be even more accurate as each division will represent 1um'.
The diameter of the reticle is important to ensure it fits the eyepiece you have.
When we select an eyepiece reticle we are only concerned with the objective magnification. The mathematics of microscope magnification is very simple ‐ all you do is divide the size of the feature on the reticle by the objective magnification to get the size that it will actually measure at the stage.
Eg. a 10mm length scale when used with a 10x objective will measure 1mm at the stage. The same 10mm length reticle when used with a 40x objective will measure 0.25mm (250µm at the stage).
If you want to look at another way, if you have a 10µm at the stage and are using a 40x objective lens this will be magnified to 400µm (0.4mm) at the reticle image plan. The next section gives more sizing information.The eyepiece merely magnifies the image on the reticle.
Table Relating Stage Size and Objective Magnification with Size at Reticle Image Plane.
The two columns on the left hand side show the size of the specimen or feature at the stage. Along the top are typical objective magnifications. So, if you want to measure something that is about 50µm using a 40x objective lens this will actually be 2mm at the reticle image plane. A reticle with a 10mm scale in 0.1mm divisions (Our NE1) or one with a 5mm scale in 0.05mm divisions (Our NE5) may be suitable.
Measurement at Stage | Measurement at Stage | 1X | 1.25X | 2X | 5X | 10X | 20X | 40X | 100X |
1m | 0.001mm | 1m | 1.25m | 2m | 5m | 10m | 20m | 40m | 100m |
5m | 0.005mm | 5m | 6m | 10m | 25m | 50m | 100m | 200m | 500m |
10m | 0.010mm | 10m | 12.5m | 20m | 50m | 100m | 200m | 400m | 1mm |
20m | 0.020mm | 20m | 25m | 40m | 100m | 200m | 400m | 800m | 2mm |
50m | 0.050mm | 50m | 60m | 100m | 250m | 500m | 1mm | 2mm | 5mm |
100m | 0.1mm | 100m | 125m | 200m | 500m | 1mm | 2mm | 4mm | 10mm |
200m | 0.2mm | 200m | 250m | 400m | 1000m | 2mm | 4mm | 8mm | 20mm |
500m | 0.5mm | 500m | 600m | 1mm | 2.5mm | 5mm | 10mm | 20mm | 50mm |
1000m | 1mm | 1mm | 1.25mm | 2mm | 5mm | 10mm | 20mm | 40mm | 100mm |
10 000m | 10mm | 10mm | 12.5mm | 20mm | 50mm | 100mm | 200mm | 400mm | 1000mm |
Colour of reticle lines does not matter in a microscope eyepiece. All lines, whatever their colour, block the light from the specimen/stage so the pattern will always appear dark against a brighter background.
Reticle Diameters for Common Microscopes
Pyser‐SGI has compiled a list of eyepieces and microscope types from the major microscope manufacturers and listed alongside them the reticle sizes that fit them. Most of these eyepieces have some means of securing the reticle in position ‐ always best to just check first before ordering the reticle. Pyser offer a supply and fit service if you have any doubts.
Brand | Eyepiece | Reticle Diameter | Model | ||
NIKON | C‐W 10xB/22 | 25mm | |||
C‐W 15x/16 | 19mm | ||||
C‐W 20x | 19mm | ||||
CFI 10x CFI 12.5x CFI 15 | 27mm | ||||
CFI UW 10x | 27mm | ||||
CFUW10 | 27mm | ||||
CFW 10x CFW 15x | 21mm | ||||
CFWN 10x CFWN 15x | 21mm | ||||
E1‐CFI 10x E2‐CFI 10x E2‐CFI 15x | 27mm | ||||
L‐W10x ESD (FOV 22) | 25mm | Eclipse LV150L | |||
SME 10x /23 | 27mm | ||||
SME 15x | ~ | ||||
SME 20x | 16mm | ||||
SME 10x /21 | 27mm | SMZ‐10 | |||
SMZ U UW10x A/24 | 25mm | SMZ‐U | |||
SMZ U UW15x | 25mm | SMZ‐U | |||
SMZ U UW20x | 21mm | SMZ‐U | |||
SMZ U UW30x | n/p | SMZ‐U | |||
SMZ 10 E10xA | 24mm | SMZ‐10 | |||
SMZ 10 UW15x /17 | 27mm | SMZ‐10 | |||
SMZ 10 UW20x | n/p | SMZ‐10 | |||
Brand | Eyepiece | Reticle Diameter | Model | ||
OLYMPUS | WHN10X / WHN10X ‐H | 24mm | BX61 / BX51 / BX41 | ||
WH10X / WH10X ‐H / WH15X | 24mm | BX50 / BX40 | |||
WHS10X‐H / WHS15X‐H | 24mm | SZX7 / SZX9 / SZX12 | |||
WHSZ10X‐H / WHSZ15X‐H | 24mm | SZ61 | |||
WHSZ20X‐H / WHSZ30X‐H | 24mm | SZ61 | |||
CWH10X / CWH10X‐H | 24mm | CX41 | |||
GSWH10X / GSWH10X‐H | 24mm | SZ60 / SZ40 / SZ11 / SD / SF | |||
GSWH15X / GSWH15X‐H | 24mm | SZ60 / SZ40 / SZ11 / SD / SF | |||
GW10X / GWH10X | 24mm | SZ60 / SZ40 / SZ11 / SD / SF | |||
WH12.5X / WH12.5X ‐H | 24mm | ||||
GWH10X‐D | 26mm | SZH / SZH10 | |||
SWHK10X | 28mm | VANOX, and other | |||
G10X / G15X | 22mm | ||||
WHK10X / WHK15X / NWHK10X | 20.4mm | 20mm loose fitting alternative | |||
(*WHK10X‐H canonot be installed) | |||||
NFK 2.5X/ 3.3X/ 5X/ 6.7X | 20.4mm | 20mm loose fitting alternative | |||
PE 2X/2.5X/ 3.3X/ 4X/ 5X | 20.4mm | 20mm loose fitting alternative | |||
GX‐SLM Scale Slider | 25.5mm | Special 3.8mm thick | |||
CWHK10X / NCWHK10X /G20X | 19mm | CK40 / CK30 / CH40 / CH30 / CH2 | |||
Brand | Eyepiece | Reticle Diameter | Model | ||
LEICA | 11507808 (S10x/25Br.M) | 26mm | DMR, DMIR, DML | ||
11507807 (S10x/22B.M) | 26mm | DMR, DMIR, DML | |||
11507801 (10x/20Br.M) | 26mm | DMR, DMIR, DML | |||
11507802 (10x/20Br.M) | 26mm | DMR, DMIR, DML | |||
11506515 (12.5x/16Br.M) | 26mm | DMR, DMIR, DML | |||
10447160 (10x/21B) | 23mm | MX series / Z series | |||
10445301 (16x/14B) | 23mm | MX series / Z series | |||
10445302 (25x/9.5B) | 23mm | MX series / Z series | |||
10445303 (40x/6B) | 23mm | MX series / Z series | |||
10450023 (10x/23B) | 24.5mm | M205C / M165C | |||
10450024 (16x /15B) | 24.5mm | M205C / M165C | |||
10450025 (25x/9.5B) | 24.5mm | M205C / M165C | |||
10450026 (40x/6B) | 24.5mm | M205C / M165C | |||
10 446 333 (10x/23,adjustable) | 24.5mm | DM750 / S6 Series / S8APO | |||
10 446 355 (16x/16,adjustable) | 24.5mm | S6 Series / S8APO | |||
10 446 357 (20x/12,adjustable) | 24.5mm | S6 Series / S8APO | |||
10 446 329 (10x/23B,adjust.f/eyeglasses) | 24.5mm | S6 Series / S8APO | |||
10 447 131 (10x/23,adjustable) | 24.5mm | S6 Series / S8APO | |||
10 447 133 (16x/16,adjustable) | 24.5mm | S6 Series / S8APO / EZ Series | |||
10 447 135 (20x/126,adjustable) | 24.5mm | S6 Series / S8APO / EZ Series | |||
10 447 137 (for eyeglasses10x/23B,adjustable) | 24.5mm | S6 Series?/S8APO | |||
10 447 139 (for eyeglasses16x/15B,adjustable) | 24.5mm | S6 Series?/S8APO | |||
10 445 302 (25x/9.5B,adjust.f/eyeglasses) | 24.5mm | S6 Series?/S8APO | |||
10 445 303 (40x/6B,adjust.f/eyeglasses) | 24.5mm | S6 Series?/S8APO | |||
10 447 280 (10x/20B,adjust.f/eyeglasses) | 24.5mm | EZ Series | |||
Leitz Wetzlar Periplan GF10x/18 | 21mm | ||||
Brand | Eyepiece | Reticle Diameter | Model | ||
ZEISS | E‐PL 10x/20 Br. Foc | 26mm | |||
PL 10x/21 Br foc | 26mm | ||||
PL 10x/23 Br. Foc | 26mm | ||||
WF 10x/18 | 23mm | Primostar | |||
PL16x/16 Br. Foc | 21mm | ||||
W 25x/10 foc | 21mm | ||||
Kpl‐W 10x/18 [#46 40 43‐9902] | 21mm | 872e | |||
Brand | Eyepiece | Reticle Diameter | Model | ||
MEIJI TECNO | EM series 10x | 25mm | |||
IM7000 series 10x | 25mm | ||||
ML2000 series 10x | 21mm | ||||
ML5000 series 10x | 21mm | ||||
ML7000 series 10x | 21mm | ||||
ML8000 series 10x | 21mm | ||||
ML8500 series 10x | 21mm | ||||
MT4000 series 10x | 21mm | ||||
MT5000 series 10x | 25mm | ||||
MT6000 series 10x | 25mm | ||||
MT9000 series 10x | 21mm | ||||
RZ series 10x | 25mm | ||||
RZ series 15x | 19mm | ||||
RZ series 20X | 19m | ||||
TC5000 seies 10x | 25mm | ||||
TM200 all series | 19mm | ||||
TM400 all series | 19mm | ||||
Brand | Eyepiece | Reticle Diameter | Model | ||
MOTIC | 100FL 10x | 21mm | |||
100FLA 10x | 21mm | ||||
100FLAQ 10x | 21mm | ||||
B1‐211 10x | 21mm | ||||
B1‐220 10x | 21mm | ||||
B1‐223 10x | 21mm | ||||
M Series 10x | 23mm | ||||
S‐10‐P 10x | 23mm | ||||
S‐20‐2L 10x | 23mm | ||||
SL‐40‐PB 10x | 23mm | ||||
SMZ‐140 10x | 23mm | ||||
SMZ‐143 10x | 23mm | ||||
ST‐30‐2L 10x | 23mm | ||||
ST‐39 10x | 23mm | ||||
10x | 24mm | BA 210, BA 310 | |||
10x | 24mm | AE 2000 | |||
Brand | Eyepiece | Reticle Diameter | Model | ||
LABOMED | Prefix 512, 312 or 913 | 21mm | |||
Prefix 912, 414, 914 or 612 | 26mm | ||||
Prefix 123, 125 or 127 | 28mm | ||||
Brand | |||||
Bausch Lomb, Cambridge Inst. | The following list shows the Bausch & Lomb reticle part number with the Pyser equivalent or nearest match. All reticles are specified as 21mm diameter. | ||||
Part Number | Description | ProSciTech Part Number | Pyser Description | ||
31‐16‐01 | Scale 5mm in 0.05mm | PYS01B21203 | NE5 Eyepiece Reticle, 5mm in 0.05mm divisions | ||
31‐16‐02 | Scale 10mm in 0.1mm. | PYS01B21201 | NE1 Eyepiece Reticle, 10mmin 0.1mm divisions | ||
31‐16‐04 | Scaled to measure 0.001" | Would be made to special order with calibration factor | |||
31‐16‐05 | Scale 5mm in 0.05mm | PYS01B21203 | NE5 Eyepiece Reticle, 5mm in 0.05mm divisions | ||
31‐16‐07 | Each least division 0.005mm @ 1x objective power | PYS01B21219 | NE31 Eyepiece Reticle | ||
31‐16‐08 | Scaled to measure 0.001" at 3x mag | Would be made to special order with calibration factor | |||
31‐16‐09 | Micro Disc | Would need image information to match pattern | |||
31‐16‐11 | 0.5mm squares | PYS01B21208OR01B21207 | NE10A Eyepiece Reticle, 10 x 10 grid of 0.5mm squares, indexed or NE10 Eyepiece Reticle, net grid of 0.5mm squares | ||
31‐16‐12 | 1mm squares | PYS01B21210OR01B21209 | NE11A Eyepiece Reticle, 10 x 10 grid of 1mm squares, indexed or NE11 Eyepiece Reticle, net grid of 1mmsquares | ||
31‐16‐71 | 100 x 1mm square grid | PYS01B21210 | NE11A Eyepiece Reticle, 10 x 10 grid of 1mm squares, indexed | ||
31‐16‐30 | Crossline, 0.080mm line width | PYS01B21234 | NE81 Crossline Eyepiece Reticle, 0.040mm line width | ||
31‐16‐42 | Scaled to measure 0.001" at 3x mag | Would need image information to match pattern | |||
31‐16‐43 | 100 divisions of 0.1mm | PYS01B21021 | NE1 Eyepiece Reticle, 10mmin 0.1mm divisions | ||
31‐16‐44 | Scaled to measure 0.01mm at 7x mag | Would be made to special order with calibration factor | |||
31‐16‐45 | Grid of 64 squares | PYS01A21218 | NE29 Eyepiece Reticle, Whipple grid | ||
31‐16‐46 | Protractor reticle, 180 deg | PYS01B21216 | NE25 Eyepiece Reticle, half protractor | ||
31‐16‐47 | General purpose reticle | Would need image information to match pattern | |||
31‐16‐87 | Stage micrometer ruled to 0.005" | PYS02A00402 | S4 Stage Micrometer, 0.1" in 0.001" divisions | ||
31‐16‐89 | Stage micrometer ruled to 0.001" | PYS02A00402 | S4 Stage Micrometer, 0.1" in 0.001" divisions | ||
31‐16‐90 | Stage micrometer ruled to 0.01mm | PYS02A00404 | S8 Stage Micrometer, 1mm in 0.01mm divisions | ||
31‐16‐99 | Stage micrometer ruled to 0.01mm | PYS02A00404 | S8 Stage Micrometer, 1mm in 0.01mm divisions | ||
31‐16‐35 | Micrometer scale 0.001" | PYS01B21214 | NE20 Eyepiece Reticle, 0.1" in 0.001" divisions |
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