Aldehyde-Fuchsin Method, pH 1.0

EMS26328

Components:

Aldehyde-Fuchsin Solution pH 1.0

Metanil Yellow Solution 0.25%

Alcohol Solution, 80% pH 1.0

Fixation:  

10% Buffered Neutral Formalin or fixative of choice.

Sections: 

Paraffin at 6µm sections

Staining Procedures:

1. Deparaffinise and hydrate to distilled water.
2. Rinse in several changes of Alcohol, 80% pH 1.0.
3. Stain in Aldehyde Fuchsin Solution for 30 minutes.
4. Rinse off excess stain with Alcohol, 80% pH 1.0.
5. Counterstain with Metanil Yellow Solution until background is a light yellow (approximately 1 minute). Rinse in distilled water.
6. Dehydrate in 95% alcohol, absolute alcohol, and clear in Xylene, two changes each.
7. Mount with Permount or DPX.

Stain Results:

All mucins except the highly acidic sulphated mucosubstances are aldehyde fuchsin negative at pH values of 1.0 and below.

References:

Johnson, W.C., Graham, J.H., and Helwig, E.B., J. Invest. Derm., 42:215-224, 1964.

Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw-Hill, c 1968, p.166