Prussian Blue Method for Haemosiderin

EMS26170, EMS26613

Components:

Potassium Ferrocyanide 2% Aqueous Solution

Hydrochloric Acid 2%

Safranin O 0.2%

Acetic Acid 1% Aqueous

Fixation:

10% Buffered Neutral Formalin

Sections:

Paraffin

Staining:

1. Deparaffinise and hydrate to Distilled Water.                            
2. Place sections in working staining solution for 30 minutes) at 60°C or for one hour  at room temperature. Rinse in distilled water.
   1. Prepare working stain by mixing equal volumes of Potassium Ferrocyanide, 2% and Hydrochloric Acid, 2%. Prepare fresh just before use.
3. Counterstain in Safranin O, 0.2% (#26170-03) for 2 minutes. Wash in Acetic Acid 1%.
4. Dehydrate in 95% Alcohol, Absolute Alcohol, clear in Xylene , and mount in synthetic resin, such as DPX.

Stain Results:

References:

Perls, M., Virchow’s Arch. F. Path. Anat., 39:42-48, 1867.

Highman, B., Arch. Path., 33:937-938, 1942.

Lillie, R.D., Histopathologic Technic and Pratical Histochemistry, 3rd. ed., McGraw Hill, N.Y., 1965

Clark, G., (ed.), Staining Procedures, 3rd ed., Williams & Wilkins, Baltimore, p. 157, c. 1973