Kinyoun's Method For Acid Fast Bacteria, Microwave

EMS26087

Components

Kinyoun's Solution

Acid Alcohol, 1%

Methylene Blue Working Solution

Section

Paraffin and frozen sections, air dried smears, touch preps.

Procedure

1. Deparaffinise sections in 2 changes of Xylene, and absolute alcohol. Air dry slides.
2. Filter 40ml Kinyoun's Carbol Fuchsin Solution, into a plastic Coplin jar. Place slides in the jar; cover with a loose screw cap. Heat in the microwave for 50 seconds.
3. Remove the Coplin jar from the oven; rinse slides individually in 50% Alcohol. Differentiate in Acid Alcohol, 1%, until sections are faint pink. Dip a few times. Rinse in 50% alcohol, then in distilled water.
4. Counterstain lightly with Methylene Blue Working Solution, for two seconds. To prepare working solution from Methylene Blue Stock Solution, mix:
   Methylene Blue Stock -10 parts
    Tap Water - 90 parts
5. Dehydrate clear in Xylene. Mount

Note: All the alcohol dilutions used in this method should be prepared with absolute 100% alcohol.

Stain Results

References

Valle, Sylvia; Journal of Histology , vol. 9, No. 4, December 1986.