Gomoris’ Methenamine Silver for Urate Crystals

EMS26614

Components:

Silver Nitrate 5%

Methenamine 30%

Sodium Borate 5%

Gold Chloride 0.1%

Sodium Thiosulfate 3%

Light Green Working Solution

Fixation:

Absolute Alcohol at 4°C for overnight.

Sections:

Paraffin @ 4-5µm

Staining Procedures:   

1. Deparaffinise to Xylene and rinse with 3 changes of absolute alcohol.
2. Place slides in preheated working* Methenamine Silver. Incubate for 30 minutes @60°C.

To prepare working solution:

Methenamine-Silver Nitrate Stock Solution:

Silver Nitrate, 5% - 5ml
Methenamine, 3% - 100ml

Methenamine-Silver Nitrate Working Solution

Methenamine Silver-Nitrate Stock Solution - 25ml
Distilled water - 25ml
Sodium Borate, 5% - 2ml
Prepare fresh prior to use. DO NOT USE IF CLOUDY. Urate Crystals should be black

1. Rinse sections in distilled water.
2. Tone with Gold Chloride, 0.1% for 5 minutes.
3. Give sections 4 or 5 rinses of distilled water.
4. Sodium Thiosulfate, 3% for 5 minutes.
5. Wash in tap water for 5 minutes.
6. Rinse in distilled water.
7. Counterstain in Light Green Working Solution for 2 minutes.
8. Dehydrate, clear and  mount with Permount or any synthetic resin

Stain Results:  

References:

Sheehan, Denza C. and Hrapchak, Barbara B. Theory and Practice of Histotechnology, 2nd ed. The C.V. Mosby Company, St. Louis, p.225 – 226, c. 1980.