Thomas' Method for Malarial Parasites

EMS26155

Components:

Phloxine B Solution 0.5%

Methylene Blue Azure B Solution

Acetic Acid 0.2% Aqueous

Fixation:

Any well fixed tissue.

Section:

Paraffin at 6µm

Staining:

1.  De-paraffinised and hydrate to distilled water.                          
2. Stain for two minutes in Phloxine B Solution, 0.5%. Rinse well in distilled water.
3. Stain for 1 minutes in Methylene Blue-Azure B Solution.
4. Differentiate in Acetic Acid 0.2%.
5. Complete the differentiation in three changes of 95% alcohol.
6. Dehydrate in absolute alcohol and clear in xylene, two changes each.
7. Mount with Permount.

Stain Results:

References:

Thomas, J.T. Stain Techn., 28:311-312, 1953

Luna, L.G. (ed.) Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw-Hill, NY., p. 129, c. 1968