Pearse's Method for Phospholipids

EMS26516

Components:

Luxol Fast Blue MBS Solution 0.1%

Lithium Carbonate 0.05% Aqueous

Neutral Red Solution 1% Aqueous

Fixation:

Bakers Calcium Cadmium Formal.

Sections:

Paraffin @ 6µm

Staining Procedures:

1. Deparaffinise and place in absolute alcohol.
2. Stain in Luxol Fast Blue Solution overnight at 58°C. Rinse in 70% Alcohol and then rinse in distilled water.
3. Differentiate for 30 minutes to 2 hours in Lithium Carbonate 0.05%. Rinse in distilled water.
4. Counterstain for 30 minutes in Neutral Red, 1%. Rinse in distilled water and blot dry.
5. Dehydrate in 95% alcohol, absolute alcohol and clear in xylene, two changes, two minutes each change.
6. Mount with Permount

Stain Results:  

References:

Pearse, A.G.E., J. Path. Bact., 70:554-557, 1995.

Luna, L.G. (ed). Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 3rd ed., McGraw-Hill, N.Y., c. 1968, p.148