Modified Verhoeff Elastic-Van Gieson Stain

EMS26369

Components

Alcoholic Haematoxylin

Ferric Chloride 2% aqueous

Lugol’s Iodine

Ferric Chloride 0.4% aqueous

Van Geisons Solution

Consistent and shorter staining time!

Fixation

10% Buffered Neutral Formalin

Sections

4-5µm

Staining

1. Deparaffinise and hydrate sections.
2. Stain in modified Verhoeff at room temperature for 7 minutes or in a 60°C oven for 3.5 minutes.
   To prepare modified Verhoeff Working Elastic solution, mix just before use:
   • 3 parts Alcoholic Haematoxylin, 3%
   • 2 parts Ferric Chloride 2%, aqueous
   •  1 part Lugol's Iodine
3. Wash in running warm tap water for 1 minute.
4. Differentiate in Ferric Chloride, 0.4% aq. for 75 seconds.
5. Wash in running warm tap water for 5 minutes.
6. Counterstain in Van Geisons solution for 60 seconds.
7. Dehydrate, clear and mount with Permount.

Results

References

Garvey, Winsome et al "A modified Verhoff Elastic Van Geison Stain" The Journal of Histotechnology, Vol. 14, No. 2 pp113-114 June 1991.