Van Gieson's Method for Collagen Fibres

EMS26046

Components:

Van Gieson's Solution

Weigert's Iron Haematoxylin A

Weigert's Iron Haematoxylin B

Fixation:

Any

Sections:

Paraffin @ 6µm or Celloidin

Staining:

1. Deparaffinise and hydrate to distilled water.
2. Weigert's Working Hematoxylin* for 10 minutes.
3. Wash in distilled water.
4. Stain 1-3 minutes in Van Gieson's Solution.
5. Dehydrate in 95% alcohol, absolute alcohol, two changes each. Clear in two changes xylene.
6. Mount with Permount or equivalents.

*Weigert's Working Solution is prepared by mixing equal parts of Weigert's Iron Haematoxylin A and Weigert's Iron Haematoxylin B

Staining Results:

Collagen: Red

Smooth and striated muscle: Yellowish to Brownish

Cornified epthelium: Yellow

Hyalin: Yellow

Nuclei: Blue to Black

References:

Clark, G., (ed.) Staining Procedures 3 rd Ed., Williams & Wilkins Co. Baltimore, c. 1973, p.50

Luna, L., (ed.) AFIP Manual of Histological Staining Methods, 3 rd Ed., New York . McGraw Hill Publication, c. 1968, p. 76.

Van Gieson, I. , New York Med. J., 50:57-60, 1889.

Mallory, F.B., Pathological Technique, W.B. Saunders Co., Philadelphia 1938.