Maldonado's Method For Pancreatic Islet Cells

EMS26458

Components

Phloxine 1% Aqueous Solution

Phosphotungstic Acid 3% Solution

Azure II Solution, 0.05%

Weigert's Iron Haematoxylin A

Weigert's Iron Haematoxylin B

Fixation

Bouin's without Acetic Acid

Section

Paraffin, @ 6µm

Staining Procedure

1. Deparaffinise and hydrate to distilled water.
2. Stain for 10 minutes in Phloxine Solution, 1% Rinse in distilled water.
3. Place for 1 minute in Phosphotungstic Acid, 3%. Rinse in distilled water.
4. Stain for 30 seconds in Azure II Solution, 0.05% Rinse in distilled water.
5. Stain in Weigert's working solution for 1 minute. Rinse in distilled water.
   To prepare Weigert's Working solution:
   Mix just before use:
   1 part Weigert's A
   1 part Weigert's B DO NOT REUSE!!!!!
6. Dehydrate in 95% alcohol, absolute alcohol and clear in xylene, two changes each.
7. Mount with Permount

Results

References

Maldonado, R. and San Jose, H. Stain Techn., 42:11-13, 1967

Luna , L.G., (ed.), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw, N.Y.
 p. 105, c. 1968