Prussian Blue Stain for Non-Haemoglobin Iron

EMS26161

Components:

Potassium Ferrocyanide 2% Aqueous Solution

Hydrochloric Acid 1%

Wright Giemsa Stain

Phosphate Buffer pH 6.5

Fixation:

None

Staining:

1. Rapidly air dry smear or imprint.                          
2. Flood slide with Wright Staining Solution.
3. Dilute the stain (on slide) with Phosphate Buffer. Leave for a length of time suitable to stain strength and cellularity of film.
4. Immerse slide in Prussian Blue Staining Solution for 10 minutes. Prepare Prussian Blue Staining Solution by mixing two parts of  Potassium Ferrocyanide 2% and three parts of Hydrochloric Acid, 1%. Prepare fresh, just before use.
5. Wash briefly in distilled water until pink colour appears, and drain dry.
6. If desired dehydrate, clear and mount with synthetic resin.

Stain Results:

References:

Clark, G. Staining Procedures, Williams and Wilkins Co. Baltimore, 3rd ed., c. 1973, p.130.

Sundberg, R., and Broman, H., Blood, 10160-6, 1955.