Giemsa Thick Film Stain

EMS26154

Components:

Giemsa Thick Film Solution
Giemsa Stock solution
Phosphate Buffer

Fixation:

None. Films are prepared by spreading 3 – 5 drops of blood on a scrupulously clean slide to form a circle of about 15mm diameter: the films are air dried by keeping in a horizontal position for 18 – 24 hours at room temperature, protected from dust and insects.

Staining:

1. Stain 40-120 minutes in Giemsa Thick Film Staining Solution.
   1. To prepare Giemsa Thick Film Staining Solution from Giemsa Stock Solution, mix before use:
      Giemsa Stock Solution - 1ml
      Phosphate Buffer - 2ml
                   Distilled Water - 47ml
2. Wash 5-10 minutes in distilled water or Phosphate Buffer
3. Dry in the air and examine.

Stain Results:

References:

Clark, G., Staining Procedures, Williams and Wilkins Co. Baltimore, 3rd, c. 1973, p. 126

Lillie, R. D. Histologic Technic and Practical Histochemistry, 3rd ed., Mcgraw-Hill, New York, 1965.

Barber, M.A., and Komp, H.W., Pub. Health Rep., 44:2330-41, 1929