Bismuth Stain Kit 3 - Mucosubstances and Polysaccharides

EMS11436

The Bismuth Stain Kit for Staining of Mucosubstances and Polysaccharides consists of:

• 10 x 10ml Solution A (Periodic acid, Ethanol, Sodium acetate in distilled water)
• 10 x 10ml Solution B (Sodium tartrate, NaOH, Bismuth subnitrate)

Procedures

1. Thin sections of conventionally fixed tissues are mounted on copper grids and floated on solution A for 10-30 minutes at room temperature.
2. After being thoroughly washed 20-30 times for 5-10 minutes with distilled water to remove periodate, the grids are floated on solution B for 30-60 minutes at room temperature. They are then again thoroughly washed with distilled water.

Reference

Ainsworth, S.K., Ito S., and Karnovsky,M.J. (1972). Alkaline bismuth reagent for high resolution ultrastructural demonstration of periodic-reactive sites. J. Histochem, Cytochem. 20, 995.