Bismuth Stain Kit 2 - Enhancing Contrast in Uranyl Acetate Treated Tissue

EMS11434

Bismuth Stain Kit for Enhancing Contrast in General consists of:

• 5 x 10ml Solution A (Sodium Tartrate in NaOH)
• 5 x 200mg Ingredient B (Bismuth Subnitrate)

Procedures

1. Drops of solution A are added into the ingredient B (Bismuth Subnitrate). The mixture is cleared after 6-8ml of solution A is added; and after the addition of all of solution A, all the Bismuth is chelated.
2. Conventionally fixed tissue specimens are stained en-bloc with a saturated Uranyl Acetate aqueous solution for 20 minutes. Thin sections on grids are stained under cover for 3 minutes by immersion in the Bismuth Subnitrate chelated above, and then rinsed with distilled water.

Reference

Riva, A. (1974). A simple and rapid staining method for enhancing the contrast of tissues previously treated with Uranyl Acetate J. Microsc. ( Paris ) 19, 105.