3-Amino-9-Ethylcarbazole Method For Leukocyte Eroxidase

EMS26173

Fixation

Formalin-Acetone Fixative

Procedure

1. Fix thin blood films in Formalin Acetone Fixative for 15 seconds.
2. Rinse gently in distilled water.
3. Stain in 3-Amino-9-Ethylcarbazole staining solution for 2.5 minutes.
4. Wash in running tap water.
5. Stain in Mayer's Haematoxylin for 8 minutes.
6. Wash in tap water, air dry, and mount in Glycerine Jelly.

Stain Results

References

Willams, W. J., Beutler, E., Erslev, A.J. and Rundles, R.W. Hematology , 2 nd ed.McGraw-Hill , NY , c 1977, pp1627-1628.