Cresyl Fast Violet, (FIRM)

EMS12780

Bridging the gap between light and electron microscopy.

Fluorophore-Infiltrated Resin Microscopy (FIRM) is a novel staining method providing ultra-high contrast imaging of tissue subcellular structure using a standard widefield fluorescence microscope. FIRM images closely resemble those obtained by transmission electron microscopy (TEM) at low magnifications. The key concept is the infiltration of thin resin sections with a fluorophore. FIRM can be used either in epoxy or acrylate resins. When illuminated by epifluorescence, the brightly fluorescent resin reveals cell and tissue structures in negative relief, according to the light absorbing and scattering properties of such structures. The method provides much greater contrast of fine subcellular structures than obtained by typical semithin section brightfield stains, such as toluidine blue. FIRM images of multiple (>50) serial semithin sections of complex structures, such as kidney glomeruli, are easily rendered in 3-D by open source software programs.

General Methods

During the testing of a large number of fluorescent dyes as candidate counterstains for thin section immunofluorescence in LR white sections, one with special properties was encountered. Most dyes, such as sodium fluorescein, non-selectively stained tissue and provided little contrast (not shown). However, one such dye, when applied to either LR White or Epon sections, provided very high contrast images using standard fluorescence microscopy, with the resin appearing brightly fluorescent and tissue structures appearing primarily in negative relief.

DISCLOSURE: James W. Mandell, MD, PhD, Dept. Pathology (Neuropathology), University of Virginia School of Medicine, Charlottesville, VA has filed a provisional patent relating to this invention: U.S. Provisional Patent Application Serial No. 61/298,759 Filed on January 27, 2010 Title: Compositions and Methods for Enhanced Specimen Contrast