Differential Quik III Stain Kit (Modified Giemsa)

Created by Nicole Smith, Modified on Mon, 11 Nov at 3:01 PM by Kathleen Patrick

EMS26096

The Differential Quik III Stain Kit is a high quality, rapid turnaround time staining kit that serves many purposes in the cost-conscious laboratory environment. The Differential Quik III Stain Kit may be used for fine needle aspirations, cytology, blood smears, bone marrow biopsies as well as bone marrow smears, touch preps, various microorgan-isms, as well as detecting the H.Pylori Microorganism. The kit contains a fixative for air-dried cell suspensions and/or touch preparation slides.

Applications:

  • Fine needle aspirations
  • Frozen Sections
  • Blood Smears
  • Cytological specimens
  • Cytopreps
  • Bone marrow biopsy
  • Touch preps
  • Microorganisms

Kit Contents:

  • Fixative -Teal
  • Solution I- Red/Orange
  • Solution II - Blue

Storage and handling:

Storage: Room Temperature
Safety: Refer to the MSDS for details.

Procedure:

  1. Fixative: Fix frozen sections, touch preps, bone marrow biopsies and blood smears for 10 seconds inFixative.
  2. Solution I-Red: Dip 5 times in Solution I
  3. Solution II-Blue: Dip 5 times in Solution II
  4. Wash in Distilled water and air dry.
  5. Quickly dehydrate in 100% EtOH,then xylene and mount in a synthetic resin.

Results:

StructureColour
ErthrocytesPink/yellowish red
PlateletsDark blue/violet/purple granules
NeutrophilsBlue nucleus, pink cytoplasm, purple granules
EosinophilsBlue nucleus, blue cytoplasm, red granules
BasophilsDark blue purple nucleus, purple granules
MonocytesPurple nucleus, light blue cytoplasm
Bacteria/microorganismsDeep blue, vary in shape
Collagen, cytoplasm & muscleVarious shades of pink, blue, orange & yellow

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